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. 2003 Feb;121(2):97–110. doi: 10.1085/jgp.20028652

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Copyright © 2003, The Rockefeller University Press

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Figure 2. — Changes in cell length, and the corresponding first and second derivative of the changes in cell length for a rigor induced contraction and relaxation. (A) At a given time, the perfusate is switched to ATP-free rigor solution, and while the cell is shortening the perfusate is switched back to ATP containing solution (arrow). After ∼150 ms the myocyte starts to relengthen, indicating completion of the perfusate switch and detachment of cross-bridges. (B) First derivative (dL·dt⁻¹) of the tracing shown in A, indicating velocity of shortening and relengthening. (C) Second derivative (dL·dt⁻²) of the tracing shown in A, indicating acceleration and deceleration of the sarcomere. All tracings were acquired at a sampling rate of 120 Hz.