Figure 1.
Liposomes aggregate in the presence of Ca2+ and annexin A4. PC:PS liposomes (20 μg lipid) were added to 2 ml buffer containing 1 mM Ca2+. Anx4 was then added in the indicated amounts and particle size was monitored continuously by quasi-elastic light scattering at 10-s intervals. 5 mM EDTA was added where indicated. Circles indicate the data points for each experimental condition after EDTA addition.