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. 2003 May;121(5):413–425. doi: 10.1085/jgp.200308803

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Immunoblot analysis of Anx4 and AQP2 in rat kidney inner medulla. (A) Samples of kidney inner medulla from control, thirsted, and hydrated rats (each lane represents an individual animal) were probed with anti-AQP2 and anti-Anx4. AQP2 is present in nonglycosylated and glycosylated forms. (B) Densitometric quantitation of protein bands in A (bars show mean ± SEM). Although the levels of AQP2 decrease dramatically with hydration, Anx4 levels remain constant during thirsting and hydration.