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. 2004 Jan;123(1):21–32. doi: 10.1085/jgp.200308916

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Copyright © 2004, The Rockefeller University Press

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Figure 6. — The COOH terminus is necessary for inactivation in spHCN channels. Currents in the absence of internal cAMP (A and C) or the presence of 100 μM cAMP (B and D) in inside-out patches expressing wt spHCN channels (A and B) and spHCN_ΔC-term channels (C and D). For the wt spHCN channels, voltage steps occurred in 10-mV increments from −10 to −120 mV, from a holding potential of −10 mV. Tails currents were at +50 mV. For the spHCN _ΔC-term channels, voltage steps occurred in 10-mV increments from −10 to −160 mV, from a holding potential of −10 mV. Tail currents were at +50 mV. The effects of the truncation of the COOH terminus of spHCN channels are: (1) the removal of inactivation, (2) a shift in the voltage dependence by −25 mV (see text), and (3) a 10-fold reduction in the expression level. (Note that spHCN channels in the excised patches activated at 20–30-mV more negative potentials than in intact oocytes, Männikkö et al., 2002.).