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. 2004 Apr;123(4):327–340. doi: 10.1085/jgp.200409031

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Copyright © 2004, The Rockefeller University Press

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Figure 9. — Effect of sulfhydryl reagents on wild-type and S79 mutant mBest2. Currents were recorded using a high intracellular free [Ca²⁺]. (A–C) Time course of the effect of MTS reagents on mBest2 currents at 100 mV. (A) Effect of 1 mM MTSET⁺ on currents induced by wild-type mBest2. (B) Effect of 10 μM MTSET⁺ (solid symbols) and 10 μM MTSES⁻ (open symbols) on currents induced by S79C mBest2. (C) Effect of 10 μM MTSET⁺ on currents induced by S79A mBest2. Note the rundown of the S79A current during the first 2 min of recording. (D) Mean effect of MTS reagents on wild-type (wt) and S79C, S79A, and S79L mBest2 currents. The ratio of the control current at +100 mV before application of the MTS reagent (I_control) and after the effect reached steady-state (I_MTS) is shown. The concentration of reagent used is shown above each bar and the number of cells tested is shown within the bar. Symmetrical Cl⁻ was used for all experiments except the experiment labeled S79C/SCN MTSET, where 140 mM Cl⁻ was replaced with SCN⁻. (E) Current-voltage relationships of S79C currents before and after MTSET⁺ in the presence of symmetrical Cl⁻ (solid lines) and with extracellular Cl⁻ replaced with SCN⁻ (dashed lines) as indicated.