TABLE I .
Effect of pH on Electrical Properties of Muscle Fibers from Adult Rats at 4 and 11 mM K+
[K+]o | [Cl−]o | pH | n | Vm | λ | Rin | Diameter | Gm | GK + | GCl − |
---|---|---|---|---|---|---|---|---|---|---|
mM | mM | mV | mm | MΩ | μm | μS/cm2 | μS/cm2 | μS/cm2 | ||
4 | 127.4 | 7.4 | 10/19 | −69 ± 1 | 0.89 ± 0.03 | 0.35 ± 0.02 | 55 ± 2 | 1017 ± 58 | ||
11 | 127.4 | 7.4 | 10/23 | −52 ± 1 | 0.65 ± 0.02 | 0.20 ± 0.01 | 62 ± 2 | 2136 ± 150 | 1731 ± 151 | |
11 | 0 | 7.4 | 9/28 | −55 ± 1 | 1.45 ± 0.06 | 0.52 ± 0.02 | 57 ± 2 | 405 ± 20 | 405 ± 20 | |
11 | 127.4 | 6.8 | 7/29 | −52 ± 1 | 0.77 ± 0.03 | 0.28 ± 0.01 | 57 ± 2 | 1393 ± 56 | 938 ± 64 | |
11 | 0 | 6.8 | 8/26 | −53 ± 1 | 1.37 ± 0.07 | 0.51 ± 0.02 | 56 ± 2 | 455 ± 30 | 455 ± 30 |
Hyperpolarizing current pulses of 75 ms duration were injected through the current electrode, and the voltage electrode recorded the membrane responses at three to five locations in each fiber. ΔVm/I ratios were plotted on a log scale against inter-electrode distance and fitted to a two-parameter exponentially decaying function, giving a straight line. The slope of the line was used to calculate the length constant, λ, and the ordinate intercept gave the input resistance, Rin (Fig. 2). Conductance was calculated from λ and Rin according to Boyd and Martin (1959) using an assumed internal resistivity (Ri) of 180 Ωcm. n gives muscles/fibers used in each group. Values are means ± SEM.