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. 2005 Feb;125(2):237–246. doi: 10.1085/jgp.200409173

TABLE I .

Effect of pH on Electrical Properties of Muscle Fibers from Adult Rats at 4 and 11 mM K+

[K+]o [Cl]o pH n Vm λ Rin Diameter Gm GK + GCl
mM mM mV mm μm μS/cm2 μS/cm2 μS/cm2
4 127.4 7.4 10/19 −69 ± 1 0.89 ± 0.03 0.35 ± 0.02 55 ± 2 1017 ± 58
11 127.4 7.4 10/23 −52 ± 1 0.65 ± 0.02 0.20 ± 0.01 62 ± 2 2136 ± 150 1731 ± 151
11 0 7.4 9/28 −55 ± 1 1.45 ± 0.06 0.52 ± 0.02 57 ± 2 405 ± 20 405 ± 20
11 127.4 6.8 7/29 −52 ± 1 0.77 ± 0.03 0.28 ± 0.01 57 ± 2 1393 ± 56 938 ± 64
11 0 6.8 8/26 −53 ± 1 1.37 ± 0.07 0.51 ± 0.02 56 ± 2 455 ± 30 455 ± 30

Hyperpolarizing current pulses of 75 ms duration were injected through the current electrode, and the voltage electrode recorded the membrane responses at three to five locations in each fiber. ΔVm/I ratios were plotted on a log scale against inter-electrode distance and fitted to a two-parameter exponentially decaying function, giving a straight line. The slope of the line was used to calculate the length constant, λ, and the ordinate intercept gave the input resistance, Rin (Fig. 2). Conductance was calculated from λ and Rin according to Boyd and Martin (1959) using an assumed internal resistivity (Ri) of 180 Ωcm. n gives muscles/fibers used in each group. Values are means ± SEM.