TABLE II.

Estimated EC₅₀ and n for the Tryptophan Mutations in TM2

Mutation	EC50 (μM)	n	Fold change in EC50 a
WT	11.8 ± 0.7	1.5 ± 0.1	1.0
T335W	1.6 ± 0.01	2.0 ± 0.01	7.4
M336W	0.8 ± 0.1	1.2 ± 0.1	14.8
I337W	1.3 ± 0.1	1.4 ± 0.2	9.1
N338W	1.5 ± 0.3	1.4 ± 0.3	7.9
V339W	1.1 ± 0.1	2.2 ± 0.6	10.7
G340W	0.3 ± 0.1	0.9 ± 0.2	39.3
S341W	NDb	ND
G342W	2.2 ± 0.2	1.7 ± 0.2	5.4
L343W	6.3 ± 0.4	1.4 ± 0.1	1.9
A344W	0.3 ± 0.06	1.0 ± 0.2	39.3
L345W	0.7 ± 0.01	1.8 ± 0.04	16.9
L346W	1.8 ± 0.3	1.3 ± 0.2	6.6
G347W	21.8 ± 3.4	1.1 ± 0.1	0.5
V348W	0.5 ± 0.1	1.2 ± 0.2	23.6
A349W	ND	ND
T350W	7.0 ± 0.3	1.4 ± 0.04	1.7
V351W	ND	ND
L352W	ND	ND
C353W	ND	ND
D354W	ND	ND
V355W	6.4 ± 0.1	1.8 ± 0.01	1.8
I356W	11.6 ± 1.8	2.0 ± 0.6	1.0
V357W	ND	ND
L358W	ND	ND

^aFold change in EC₅₀ was calculated as EC₅₀(WT)/EC₅₀(mutant).

^bND, not determined.