Table 2.

Oligonucleotide primers used for RT-PCR of cyclin D1, GST-P, Bcl2, Bax and VEGF with β-actin as an internal control

Gene Product	Primers	Oligonucleotide sequences	Fragment size
Cyclin D1	Sense Antisense	5'-CGGAGGACAACAAACAGATC-3' 5'-GGGTGTGCAAGCCAGGTCCA-3'	331 bp
GST-P	Sense Antisense	5'-TCATCTACACCAACTATGAG-3' 5'-GCCACATAGGCAG AGAGCAG-3'	226 bp
Bcl-2	Sense Antisense	5'-TGCACCTGACGCCCTTCAC-3' 5'-AGACAGCCAGG AGAAATCA AACAG-3'	293 bp
Bax	Sense Antisense	5'-ACCAAG CTGAGCGA GTGTC-3' 5'-ACAAAGATGGTCACGGTCTGCC-3'	374 bp
VEGF	Sense Antisense	5'-ATGAACTTTCTGCTGTCTTGG-3' 5'-TCACCGCCTCGGCTTGTCACA-3'	444 bp and 576 bp*
β-actin	Sense Antisense	5'-AACCGCGAGAAGATGACCCAGATCATGTTT-3' 5'-AGCAGCCGTGGCCATC TCTTGCTCGAAGTC-3'	350 bp

*The primers for VEGF detect two of four different molecular species produced by alternative splicing of mRNA-VEGFI21 and VEGFI65, with expected fragment sizes of 444 bp and 576 bp, respectively.