Table 3.

Tumour incidence, tumour burden and histopathological changes in the buccal pouch of experimental animals (mean ± SD; n = 6)

Group	Treatment	Tumour incidence	Tumour burdena mm3	Hyperplasia	Dysplasia	Squamous cell carcinoma
1.	DMBA	6/6	127.21 ± 87.42	+++	+++	# (100%)
2.	DMBA+ P-B	1/6 b	8.31 ± 7.92c	++	+/++	# (16.6%)b
3.	DMBA+ BTF-35	0/6d	-	+	-	-
4	Control	0/6	-	-	-	-

+ = mild; ++ = moderate; +++ = severe; # = well differentiated; - = no change; Parentheses, percentage of lesions

a – Mean tumour burden was calculated by multiplying the mean tumour volume with the mean number of tumours (tumour volume was calculated using 4/3 πr³, where r = 1/2 tumour diameter in mm)

b – Significantly different from group 1 by χ²-test (p < 0.05)

c – Significantly different from group 1 by Student's t test (p < 0.001)

d – Significantly different from group 1 by χ²-test (p < 0.001)