Table 4.

Cellular localization of p21 and expression of GST-P, NF-κB, cytokeratin and VEGF in the buccal pouch of experimental and control animals (n = 6)

Group	Treatment	Cellular localization of p21 protein		p21a				GST-Pa				NF-κBa				Cytokeratinb			VEGFa
		Cytoplasm	Nucleus	0	1	2	3	0	1	2	3	0	1	2	3	I	II	III	0	1	2	3
1.	DMBA	85%c	<5%c	0	0	2	4	0c	0	1	5c	0c	0	1	5c	0	0c	6c	0	0	0	6c
2.	DMBA+P- B	5%d	73%d	0	0	3	3	3	1	2	0d	0	1	5	0d	0	4	2	0	3	3	0d
3.	DMBA+BTF-35	<5%d	75%d	0	0	2	4	4	2	0	0d	0	4	2	0d	0	5d	1d	0	5d	1	0d
4.	P-B	-	-	6	0	0	0	6	0	0	0	6	0	0	0	0	6	0	0	3	3	0
5.	BTF-35	-	-	6	0	0	0	6	0	0	0	6	0	0	0	0	6	0	0	2	4	0
6.	Control	-	-	6	0	0	0	6	0	0	0	6	0	0	0	0	6	0	0	2	4	0

a – Expression scored as 0- negative; 1- focal and mildly intense; 2- between one-third and two-third of cells stained moderately, and 3- majority of cells (> two third) stained intensely

b – Expression graded as 0 = failure to detect the keratin, I = staining confined either to the basal area or some evidence of suprabasal staining, II = positive staining throughout the basal and/or suprabasal region.

c – Significantly different from group 6 by χ²-test (p < 0.05)

d – Significantly different from group 1 by χ²-test (p < 0.05)