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. 2007 Nov 13;2:25. doi: 10.1186/1749-8104-2-25

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Copyright © 2007 Guinazu et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Prechordal-epichordal interactions anterior to the MHB result in ZLI induction in vivo. (A) Ectopic induction of Shh following transplantation of quail pro-Pth tissue into chick midbrain at stage HH10. (B) QCPN antibody staining allowed for localisation of the graft (asterisk in (A)). (C) Ectopic induction of Shh following transplantation of quail telencephalic tissue into chick midbrain at stage HH10. (D) QCPN antibody staining allowed for localisation of the graft (asterisk in (C)). (E) A graft of quail midbrain tissue into a chick pro-Th at stage HH10 maintains midbrain identity (red arrowhead; Dmbx1 expression in blue), but does not result in ectopic induction of Shh (red).