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The ZLI is necessary and sufficient for the expression of thalamic differentiation genes in explant culture. (A) Pro-Pth and (B) pro-ZLI + pro-Pth explants were isolated from stage HH10 embryos, cultured for 72 hours and analysed for the expression of Shh (red) and Dlx2 (blue). (C) Pro-Th and (D) pro-ZLI + pro-Th explants were isolated from stage HH10 embryos, cultured for 72 hours and analysed for the expression of Shh (red) and Gbx2 (blue).
