Table II.

Examples of shRNAs Targeting HBV

Gene	Promoter	Vector	Host	Transfection/Delivery	Results	Reference
C, S, P, X	Human U6	Plasmid	Mice	Hydrodynamic injection	77–92% reduction of HBV DNA and RNA	[12]
					85–99% reduction of HBsAg and HBcAg
C, X	Human H1	Plasmid	Huh7 or HepG2.2.15 cells	Calcium phosphate	68–95% reduction of HBV DNA and RNA	[98]
					63–89% reduction of HBeAg
C, S, P, X also DR	Human U6	Plasmid	HepG2 cells	Lipofectamine 2000	90–97% reduction of HBV DNA and RNA	[101]
S	Human U6	Plasmid	HepG 2.2.15 cells	Lipofectamine 2000	44% reduction of HBsAg	[102]
La	Human U6	Plasmid	HepG2 and 2.2.15 cells	Lipofectamine 2000	8–66 reduction of HBV RNAs	[110]
					26–51% reduction of HBsAg and HBeAg
S	Human U6	Plasmid	HepG2 and 2.2.15 cells	Lipofectamine 2000	90% reduction of HBsAg-GFP fusion gene	[111]
					43–64% reduction of HbsAg and HBeAg
C, S, P, X also DR	Mouse U6	Plasmid	HepG 2.2.15 cells	Lipofectamine 2000	2 reduction of HBV DNA	[104]
					56–72% reduction of HbsAg and HBeAg
S	Human H1 and U6	Plasmid	Mice	Lipofectamine 2000	Reduction of HBV DNA, RNA, HBsAg, HBcAg	[105]
P, S, X	Human H1	Plasmid	Mice	Hydrodynamic injection	95–99% reduction of HBsAg and HBcAg	[97]
					Rare mutation allowed escape from shRNA
S	Human H1	Plasmid	HepG 2.2.15 cells	Lipofectamine 2000	80–90% reduction of HBsAg mRNA	[103]
					71–98% reduction of HBsAg
C, S, P, X	Human U6	Plasmid	Huh-7 cells	Adenoviral transduction	84% reduction of HBsAg	[126]
					99% reduction of HBcAg and HBeAg
S, P, X	Mouse U6	1st g Adv	Mice	Adenoviral transduction	>90% reduction of HBV DNA and RNA	[99]
					Up to 100 reduction of HBsAg and HBcAg

Abbreviation: C, core antigen; S, surface antigen; P, polymerase; S, X protein; La, human La protein; PFV, prototype foamy virus; AAV, adeno-associated virus, 1st g Adv, first generation adenovirus.