. 2007 Dec 11;13(2):225–235. doi: 10.1007/s10495-007-0166-5

Table 1.

Characteristics of biotinylated TRAIL variants

	Relative biotin content (Biotin/TRAIL)^a	Activity in cell viability assay (IC₅₀ in ng/ml)^b	Activity in caspase 3/7 assay^c (%)
TRAIL	–	40	100
Bio-NHS-TRAIL	1.6	123	35
Bio-ULS-TRAIL	0.8	60	126

^aExpressed as mole biotin bound per mole of trimeric TRAIL. Relative biotin content was determined by MALDI-TOF analysis (bio-NHS-TRAIL) or ELISA (BIO-ULS-TRAIL)

^bAs determined after 48 h of incubation with Jurkat cells

^cAs determined after 4 h of incubation at 10 ng/ml with Jurkat cells