Figure 8.

Distribution of Phosphorylated Histone H3 in Plants Containing Two Minichromosomes 3.

The DAPI-stained chromosomes are blue; the polyclonal antibody raised against phosphorylated Ser-10 to histone H3 is magenta; the ZmBs is green. (A) to (D) show metaphase II, and (E) to (H) show anaphase II. The sister cells are not shown. There is no difference in the distribution of phosphorylated histone H3 at Ser-10 between the normal chromosomes and the minichromosome. (A) and (E) show merged images, (B) and (F) show DAPI, (C) and (G) show B repeat plus DAPI, and (D) and (H) show histone phosphorylation plus DAPI. Bar = 10 μm.