Figure 1.

The Maize cfm2 Gene.

(A) Domain organization of the CFM2 protein. Regions of similarity with CRS1 are represented with like shading. The fragment used for antisera generation is marked.

(B) Schematic of the major maize cfm2 mRNA. Exons are indicated with boxes and introns with dotted lines. Exons in black were represented in cDNAs. The gray exon segment at the 5′ end was inferred from genomic DNA sequence. The exons represented in the cDNA probes used for DNA and RNA gel blots are indicated.

(C) RNA gel blot hybridization showing cfm2 mRNA. Seedling leaf RNA (polyA+, polyA-, or total) was hybridized to cDNA probe B diagrammed in (B). The same blot stained with methylene blue is shown below, with rRNA bands marked.

(D) DNA gel blot hybridization suggesting the presence of two cfm2 genes in maize. Total leaf DNA digested with the indicated enzyme was hybridized with cDNA probes A or C, diagrammed in (B). The blot was washed at high stringency (65°C, 0.2× SSC). The genomic regions corresponding to these probes lack sites for the enzymes used to digest the DNA.