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. 2007 Dec;19(12):3864–3875. doi: 10.1105/tpc.107.055160

Figure 2.

Figure 2.

Maize CFM2 Is Localized to the Chloroplast Stroma.

Chloroplasts (Cp) and chloroplast subfractions were from the fractionated chloroplast preparation described previously (Williams and Barkan, 2003). Equivalent proportions of each fraction were examined by immunoblot analysis using antisera specific for the protein indicated to the left. The same blot stained with Ponceau S is shown below. Cpn60, PsbA, and IM35 were used as markers for the stroma, thylakoid, and inner envelope, respectively. The major band detected with the CFM2 antibodies corresponds in size to the CFM2 protein with four CRM domains that is conserved with the gene models of the Arabidopsis and rice orthologs. The asterisk denotes a second protein detected by affinity-purified antibodies from two different rabbits immunized with the CFM2 antigen. Env, envelope; Str, stroma; Thy, thylakoid.

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