Figure 6.
Viability, Germination, and Elongation of Slr1-d3 Pollen.
(A) and (D) Viability of wild-type T65 (A) and Slr1-d3 (D) pollen. Pollen grains were stained with I2-KI solution. Pollen grains staining black were judged as viable, and those staining yellow or light red were considered sterile.
(B), (C), (E), and (F) Growth of pollen of the wild type ([B] and [C]) and Slr1-d3 ([E] and [F]) on their own stigmas. Pollen was stained with aniline blue after 30 min ([B] and [E]) or 2 h ([C] and [F]). Artificial pollination was performed by hand. Arrowheads indicate pollen elongation.
Bars = 200 μm.