Figure 2.

Wild-Type Type-A ARR Overexpression Confers Cytokinin Resistance.

Overexpression of ARR4, ARR5, ARR6, ARR7, and ARR9 confers cytokinin resistance.

(A) Seedlings were grown as described for Figure 1E with the specified concentrations of BA or 0.1% DMSO control.

(B) Transgenic seedlings express myc-tagged ARR proteins, detected as in Figure 1E. Bands corresponding to the relevant protein products quantified in Figure 3 are noted with asterisks. Lower molecular mass bands in ARR4 and ARR6 may represent degradation products. Arrows indicate the positions of 47.5- and 32.5-kD protein markers.

(C) Root elongation was measured as described for Figure 1. Error bars represent se (n > 30). Asterisks indicate statistically significant differences from the wild type at the given concentrations of BA (Student's t test, P < 0.05).