Figure 5.

ARR5 and ARR7 Protein Stability Is Dependent on the Conserved Phosphorylation Target Asp.

Seedlings expressing the proteins indicated were grown, treated, and analyzed as described for Figures 3D and 3E. In (C) and (G), asterisks indicate significant differences in relative protein levels from ARR5^WT or ARR7^WT after the same treatment (Student's t test, P < 0.05). The data for triplicate analysis of ARR7^WT protein degradation is presented in Figure 3E. In (D), genomic versions of myc-ARR5 were first induced by cytokinin to elevate myc-ARR5 protein levels before treatment with CHX.