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. 1999 Jul 6;96(14):8007–8012. doi: 10.1073/pnas.96.14.8007

Figure 3.

Figure 3

Photoprotection and thermally induced photoinhibition in cultured symbiotic dinoflagellates. (A) The quantum yield of PSII without (○, ●) and with (□, ■) the addition of lincomycin for Symbiodinium sp. from the giant clam T. gigas grown at 26 (open symbols) and 32°C (closed symbols) (n = 6 for each temperature). (B) The quantum yield of PSII for Symbiodinium sp. isolated from the Caribbean coral O. diffusa grown at 26 and 32°C with and without the addition of lincomycin (symbols same as for A). (C) Relationship between PSII efficiency and the amount of D1 protein (Inset) in dinoflagellates from T. gigas grown at 26 (□) and 32°C (■) with chloramphenicol and at 26°C (○) without the inhibitor (n = 3). Similar results were found with lincomycin, as well. (D) qN and qP of chlorophyll fluorescence for Symbiodinium sp. from T. gigas and O. diffusa grown at 26 and 32°C for 7 days. (E) Net content of the D1 protein within the dinoflagellates after 7 days of exposure to 26 and 32°C (n = 3). (F) Representative immunoblots of the D1 protein from Symbiodinium sp. from T. gigas grown at 26 and 32°C for 7 days and Symbiodinium sp. from O. diffusa grown at 26 and 32°C for 7 and 10 days. (Bars = SD.)