Figure 4.
Oligomycin-dependent phenotype of Δnop-1 N. crassa strains and retinal binding of NOP-1 heterologously expressed in P. pastoris. (A) Sensitivity to oligomycin. Wild-type and Δnop-1 N. crassa strains were cultured on 100 ng/ml oligomycin-containing medium in constant light or darkness for 4 days at room temperature and then photographed. (B) Binding of all-trans retinal to NOP-1. Crude membrane fractions from P. pastoris strains containing nop-1 overexpression (+) or control (−) plasmid were incubated with all-trans [3H]retinal, the linkage reduced by sodium cyanoborohydride, and samples analyzed by using SDS/PAGE followed by fluorography. The position of the NOP-1 protein is indicated by the arrow.