Figure 3.

(A) Transcription profile of DAZ transgene in a range of tissues (lane 1, ovary; lane 2, testes; lane 3, female brain; lane 4, male brain; lane 5, female kidney; and lane 6, male kidney) from TgS12 transgenic mice assayed by RT-PCR. DAZ and S16 ribosomal protein primers described in Materials and Methods. (B) Northern hybridization to adult testis RNA of wild-type mouse (lane 1), TgS12 transgenic mouse (lane 2), and human (lane 3) with probe DAZ3TER (Upper). The diffuse signal in human RNA represents a range of DAZ transcripts containing different numbers of the 24-aa DAZ repeat. There is no apparent cross-hybridization to mouse Dazl. Signal obtained with a mouse actin probe is shown (Lower).