Figure 5.

(A) Structural rearrangement of yS12 resulting in fusion of HSV-TK and DAZ sequences. Positions and orientations of the DAZ gene and the LYS2, TRP1, and HSV-TK genes within the modified YAC are indicated. (B) Interstitial deletion has resulted in a fusion of HSV-TK sequence to the DAZ gene with elimination of DAZ exon 1 (including initiation codon) and intron 1. The location of a potential initiation codon is indicated by an asterisk. (C) Predicted amino acid sequence of the fusion transcript. DAZ nucleotide and amino acid sequences (in B) and amino acids introduced by fusion (in C) are underlined.