Pictures of colonies of different strains grown in casitone-Tris plates with 1.5% or 0.5% agar. Drops of cells (2 μl) were deposited on the agar. After the drops had dried, the plates were incubated at 33°C for 4 days. The motility phenotype of the strains is indicated. wt, DK1622; A−S−, cglB, pilA; A−frz−, aglB1, frzE; A−, aglB1; S− frz−, pilA frzE; frz−, frzE; S−, pilA.