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. 1977 Nov;132(2):426–433. doi: 10.1128/jb.132.2.426-433.1977

Isolation and Properties of Two Classes of Low-Density Vesicles from Saccharomyces cerevisiae

T G Cartledge 1,†, A H Rose 1, Diane M Belk 1, Adelina A Goodall 1
PMCID: PMC221881  PMID: 334741

Abstract

A mixture of small (0.43-μm diameter) and large (0.62-μm diameter) low-density vesicles from spheroplasts of Saccharomyces cerevisiae was fractionated by rate centrifugation in a gradient of 0 to 8% (wt/vol) Ficoll to yield fractions rich (90 to 95%) in small or large vesicles. The large, but not small, vesicles swelled when diluted into mannitol solutions containing less than 0.4 M mannitol. The pH-electrophoretic mobility curve of the large vesicles showed that they are probably enclosed in a phospholipid-protein membrane. The dyes neutral red and toluidine blue, accumulated into large vesicles by intact cells and spheroplasts, were largely lost from large vesicles when these were separated from stained spheroplasts. Sudan black III stained small and large vesicles, both classes of vesicle retaining the stain on separation. Fractions rich in large vesicles contained proportionately more phospholipid and less free sterols, diacylglycerols, and free fatty acids compared with those enriched in small vesicles. The two classes of vesicles contained about the same proportions of esterified sterols and triacylglycerols. The free fatty acids in both small and large vesicles were free from unsaturated fatty-acyl residues; diacylglycerols and triacylglycerols contained appreciable proportions of unsaturated fatty-acyl residues. Small vesicles were richer in lipase activity, whereas the larger vesicles contained greater β-glucanase and α-mannosidase activities. Phospholipase activity could not be detected in any of the fractions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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