Table 2.
List of primers used in PCR experiments.
| Primer | Primer sequencea | Gene to be amplified | Template |
|---|---|---|---|
| Primer 1 | aaaaccgttgatatctttgcaaacgggcatgactcctgactttt | aldA promoter | E. coli W3110 chromosome |
| Primer 2 | aaaaccgttgaattcctcctgtgatttatatgtttgttttc | ||
| Primer 3 | aaaaccgttgatatctgcagaatgaagggtgatttatgtgatttg | udp promoter | E. coli W3110 chromosome |
| Primer 4 | aaaaccgttgaattcctcctctgtgaatcggtttagtcaga | ||
| Primer 5 | ggaattcatgagtaaaggagaagaacttt | GFP | pGFPuv |
| Primer 6 | cccaagcttttatttgatgagctcatcc |
aRestriction enzyme sites are shown in bold.