Figure 1.

αA-crystallin immunoreactive proteins present in mouse C2C12 cells after stable transfection with the hamster αA-crystallin gene. (A) Immunoblot after SDS/PAGE of total extracts from transfected cells with a high expression level of normal αA- and αA^ins-crystallin with masses of 19.8 and 22.5 kDa, respectively (line pVim-αA11; left lane) and transfected cells expressing two additional αA-crystallin-like proteins: super αA-crystallin and super αA^ins-crystallin (line pVim-αA7; right lane). Detection was achieved with a polyclonal anti-αA-crystallin antiserum. (B) Immunoblot after two-dimensional electrophoresis of cell extract from line pVim-αA7. Detection was achieved with a monoclonal anti-αA-crystallin antiserum. Arrowheads indicate presumed monophosphorylation products. The different ratios between the four αA-crystallin-positive proteins in A and B resulted from the use of different antisera and staining conditions.