Figure 3.

Complex size of α-crystallin expressed in transfected mouse C2C12 cells. (A) Elution profiles after size-exclusion chromatography (Superose 6) of (i) calf lens soluble proteins, (ii) extract of cell line 7, and (iii) extract of cell line 11. Calf lens α-crystallin complexes have a mass of ≈700 kDa. (B) Western blotting after SDS/PAGE of Superose 6 fractions from cell line 7 (Upper) and cell line 11 (Lower). Lanes are numbered according to the elution times shown in A. Blots are stained with a monoclonal anti-αA-crystallin antiserum.