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. 1999 Jul 6;96(14):8080–8085. doi: 10.1073/pnas.96.14.8080

Figure 2.

Figure 2

Concentration-dependency of IL-4 and IL-4 inhibitor effects on MC growth and effect of IL-4 on histamine content and MC subtypes. (A) Concentration-dependent effect of IL-4 on MC proliferation (culture time 19–21 days, n = 4). (B) Inhibition of the IL-4 (1 ng/ml) effect on MC recovery by RY, a competitive IL-4 inhibitor (expressed in percent, MC number without IL-4 and without RY was set 100%, n = 3). (C) Total cellular histamine content per MC determined after cell lysis (n = 8) in MC cultured for different time periods. (D) MC subtypes assessed by immunocytochemistry (MCT, tryptase-positive MC; MCTC, tryptase/chymase-double-positive MC; Tryp−, tryptase-negative cells). Total cell numbers at start of culture were set 100%. Data obtained after culture (17–21 days, ± IL-4) are expressed in percent of total cell number at time of start of culture, n = 5; ∗, P < 0.05 compared with percentages at culture start and after culture without IL-4).