Figure 6.
Effect of IL-4 on MC mediator release and cytokine production induced by IgE receptor crosslinking. (A) MC were cultured in medium supplemented with SCF ± IL-4 for 7–21 days, washed, and then challenged for 30 min (histamine, LTC4) or 6 hr (TNF-α, IL-5) with mAb 29C6 (100 ng/ml) directed against the α-chain of the high-affinity IgE receptor. Specific mediator release into supernatants is shown. Each symbol represents one experiment performed in duplicate. Data derived from the same experiment (±IL-4) are connected by lines. Time course of IL-4 in altering (B) histamine release and (C) LTC4 production in MC. Cells were cultured with or without IL-4 for different time periods and then triggered for 30 min by IgE receptor crosslinking (one of two experiments performed in duplicate is shown).