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IA-2 transfected mouse insulinoma MIN-6 cells undergo growth arrest in high glucose media. (a, b) Growth rate of IA-2 and mock transfected MIN-6 cells as determined by (a) trypan blue staining and (b) bromodeoxyuridine (BrdU) cell proliferation. (c, d) Cell cycle analysis by flow cytometry of mock and IA-2F transfected MIN-6 cells under (c) low and (d) high glucose conditions. (e) Survivin expression in mock and IA-2F transfected MIN-6 cells as determined by Western blot.
