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. 2007 Oct;150(1):49–60. doi: 10.1111/j.1365-2249.2007.03455.x

Fig. 3.

Fig. 3

IA-2 siRNA treatment prevents G2/M arrest and apoptosis in IA-2 transfected mouse insulinoma MIN-6 cells. (a) IA-2 expression as determined by Western blot 48 h after transfection with IA-2 siRNA and non-silencing siRNA. (b, c) Cell cycle analysis of IA-2F transfected MIN-6 cells as determined by flow cytometry 48 h after transfection with IA-2 siRNA or non-silencing siRNA in low and high glucose media. (d, e) Detection of apoptotic cells by flow cytometry 72 h after exposure of IA-2F transfected MIN-6 cells to IA-2 siRNA and non-silencing siRNA in low and high glucose media. (f, g) Long-term passage of IA-2F transfected MIN-6 cells kicks out IA-2 as shown by (f) Western blot and (g) when this occurs (e.g. by the 18th passage) glucose-induced G2/M arrest is prevented.