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. 2007 Oct;150(1):49–60. doi: 10.1111/j.1365-2249.2007.03455.x

Fig. 4.

Fig. 4

Phosphorylation of proteins involved in the insulin signalling pathway. (a) Phosphorylation of Akt/protein kinase B (PKB), phosphoinositide-dependent kinase (PDK)-1, mammalian target of rapamycin (mTOR), glycogen synthetase kinase (GSK)-3β, p70S6 kinase (p70S6K), phosphatidylinositol phosphate 3'-phosphatase (PTEN), PI3 kinase (PI3K), insulin receptor substrate (IRS)-1 and insulin receptor (IR) in mock and IA-2F transfected mouse insulinoma MIN-6 cells in high and low glucose media. (b) Phosphorylation of Akt/PKB in IA-2F transfected MIN-6 cells, or (c) non-transfected MIN-6 cells in the presence of high glucose 48 h after exposure to IA-2 siRNA and non-silencing siRNA. (d) Apoptosis as measured by flow cytometry with anti-annexin V and propidium iodine in the presence of tumour necrosis factor (TNF)-α and interferon (IFN)-γ at low or high glucose concentrations in mock and IA-2F transfected MIN-6 cells. Mean ± s.e. of three separate experiments. *P < 0·05, **P < 0·01.