Skip to main content
. 2007 Oct;150(1):49–60. doi: 10.1111/j.1365-2249.2007.03455.x

Fig. 5.

Fig. 5

Sorting nexin (SNX)19 interacts and co-localizes with IA-2 and SNX19 siRNA increases Akt/phosphoinositide-dependent kinase (PDK)-1 phosphorylation and inhibits G2/M arrest and apoptosis. (a) Lysates of NIH3T3 cells co-transfected with anti-flagellar antigen (FLAG)-tagged SNX19 and c-Myc-tagged IA-2IC (a.a. 600–979) were immunoprecipitated with anti-FLAG antibody, anti-c-Myc antibody or anti-IA-2 antibody followed by Western blot analysis. (b) Endogenous interaction of IA-2 and SNX19 in mouse insulinoma MIN-6 cells (non-transfected) detected by immunoprecipitation with anti-IA-2 or anti-SNX19 antibody followed by Western blot. (c) Fractionation of lysates from non-transfected MIN-6 cells followed by Western blot analysis with anti-IA-2, -SNX19 and -insulin antibody. (d) MIN-6 cells were transiently transfected with SNX19 and analysed 48 h later by flow cytometry for apoptosis in the presence of high glucose. (e) PC12 cells were transiently transfected in the presence of high glucose with SNX19 or co-transfected with SNX19 and IA-2 and 48 h later Akt/PKB phosphorylation was determined by Western blot. (f) Non-IA-2 transfected MIN-6 cells were transfected with SNX19 siRNA or non-silencing siRNA and Akt/protein kinase B (PKB) phosphorylation determined by Western blot. (g h) IA-2F transfected MIN-6 cells were transfected with SNX19 siRNA or non-silencing siRNA and the effect on (g) cell cycle and (h) apoptosis was determined by flow cytometry in the presence of high glucose.