Intensity of VEGF-A expression after LPrA treatment 7 d after uterine tissue transfer. Native endometrium VEGF-A expression was similar between control (Ctl) (A), LPrA- (B), and LPrASc- (C) treated groups. Marked reduction on ectopic lesions was demonstrated in the LPrA- (E) treated group compared with control (D) and LPrASc-treated animals (F). Quantification and comparison of VEGF-A expression intensity. G, Ten random HPFs per replicate were selected, and stromal and epithelial intensity was quantified with grading as follows: marked intensity (2++) moderate intensity (2+), low intensity (+), trace intensity (tr), and no staining (0). Endometriosis-like lesion VEGF-A expression intensity of LPrA-treated animals (trace to no staining) was reduced 2-fold when compared with control and LPrASc-treated animals (2+). Scale bars, 100 μm.