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. 2007 Oct 25;149(2):506–514. doi: 10.1210/en.2007-1225

Figure 6.

Figure 6

Immunohistochemical analysis of CD 31 in murine endometriosis-like lesions after pegylated leptin peptide antagonist (LPrA) treatment. Inverted epifluorescent filtered microscopy images for the CD 31 secondary antibody (wavelength 594 nm) were used. Images include CD 31, Hoechst, and merged (CD 31 and Hoechst layered) for LPrASc (A–C) and LPrA (D–F) for continuous (Cont) treatment courses and acute time courses for LPrASc (G–I) and LPrA (J–L). Images demonstrated markedly reduced CD 31 fluorescent intensity in LPrA-treated endometriosis-like lesions compared with LPrASc in both the continuous and acute treatment time courses. Quantification of MVD in endometriosis-like lesions. M, Three random HPFs (20×) per sample were selected, and the mean MVD (percentage of HPFs) from CD 31 secondary Ab stain was assessed. Mean percentage ± sem for each group (n = 3 mice per group) was calculated. Respective values were compared using ANOVA and post hoc Tukey test. Different letters represent differences at the level of P < 0.01. Scale bars, 100 μm.