. 1999 Jul 6;96(14):8092–8097. doi: 10.1073/pnas.96.14.8092

Table 1.

H-2 restriction of nude T cells after reconstitution with thymus grafts without or together with immunodefective bone marrow-derived cells

Exp.	Recipient^*	Donor		Specific lysis of targets, (%)
Exp.	Recipient^*	Thymus	Bone marrow- derived cells	MC57 (H-2^b)		P185 (H-2^d)
				LCMV	Uninf.	p118	Uninf.
1	Nude (H-2^b × H-2^d)	H-2^b	None	84	4	15	6
	(C57BL/6 × BALB/c) F1	RAG^0/0		74	3	5	7
		H-2^d	None	19	10	64	6
		SCID		11	1	70	3
		H-2^d	None	2	2	56	1
		RAG^0/0		<1	<1	46	2
				MC57 (H-2^b)		L929 (H-2^k)
2	Nude (H-2^b) C57BL/6 (>95%)	H-2^k	None (<8%)	69	7	14	15
		RAG^0/0		52	5	11	12
				35	1	7	7
				24	2	5	4
	Nude (H-2^b) C57BL/6 (>95%)	H-2^k	None	99	14	19	25
		RAG^0/0		77	7	12	14
				42	1	4	14
				32	3	3	17
	Nude (H-2^b) C57BL/6 (60%)	H-2^k	H-2^k (40%)	23	35	70	19
		RAG^0/0	RAG^0/0	14	11	74	22
				7	5	69	14
				9	2	44	8
	Nude (H-2^b) C57BL/6 (50%)	H-2^k	H-2^k (50%)	68	11	71	35
		RAG^0/0	RAG^0/0	49	5	67	24
				27	3	60	16
				12	1	44	12
	Controls:
	C3H (H-2^k)			16	12	76	30
	C57BL/6 (H-2^b)			97	9	32	27

The data shown are representative for three comparable experiments for Exp. 1, a total of four to eight mice each tested in Exp. 2 and 3. uninf., uninfected.

Mice were infected with 10² pfu of LCMV; 7 or 8 days later, CTL activity was tested in a 5-h assay on the indicated targets at spleen cell-to-target cell ratios of 90, 30, 10, or 3:1 [except for Exp. 1 and controls, where only 90 and 30 or 90 are shown (top to bottom)]. Bold values are significant above relevant controls (p < 0.1). Numbers in parentheses are % of lymphocytes of the indicated H-2.