Table 2.
H-2 restriction of nude T cells after reconstitution with thymus grafts without or together with immunodefective bone marrow-derived cells
| Exp. | Recipient* | Donor
|
Specific lysis of targets, (%)
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Primary response
|
Secondary response after restimulation with
|
||||||||||
| C57BL/6 (H-2b)
|
ICR (H-2q)
|
||||||||||
| MC57 (H-2b)
|
DBA1 (H-2q)
|
MC57 (H-2b)
|
DBA1 (H-2q)
|
||||||||
| Thymus | Bone marrow- derived cells | LCMV | Uninf. | p118 | Uninf. | LCMV | Uninf. | p118 | Uninf. | ||
| 3 | Nude (H-2q) | H-2b | None | <1 | 23 | 80 | <1 | 9 | <1 | 88 | 27 |
| ICR (>95%) | RAG0/0 | <1 | 16 | 55 | <1 | 5 | <1 | 90 | 11 | ||
| 3 | 1 | 35 | <1 | 3 | <1 | 68 | 3 | ||||
| <1 | <1 | 13 | <1 | 3 | <1 | 41 | <1 | ||||
| Nude (H-2q) | H-2b | H-2b | 70 | <1 | 80 | <1 | 92 | <1 | 86 | 26 | |
| ICR (70%) | RAG0/0 | RAG0/0 (30%) | 45 | 2 | 80 | 2 | 67 | <1 | 71 | 6 | |
| 38 | <1 | 58 | <1 | 40 | <1 | 49 | 3 | ||||
| 17 | <1 | 34 | <1 | 18 | <1 | 25 | 3 | ||||
| Controls: | |||||||||||
| ICR (H-2q) | 6 | 2 | 69 | <1 | † | 82 | 105 | 27 | |||
| C57BL/6 (H-2b) | 81 | 12 | 27 | 24 | 100 | 30 | † | 96 | |||
The data shown are representative for three comparable experiments for Exp. 1, a total of four to eight mice each tested in Exp. 2 and 3; uninf., uninfected.
Mice were infected with 102 pfu of LCMV; 7 or 8 days later, CTL activity was tested in a 5-h assay on the indicated targets at spleen cell-to-target cell ratios of 90, 30, 10, or 3:1 (top to bottom). Secondary restimulation in vitro for Exp. 3 was for 5 days with infected indicated irradiated splenocytes as stimulator cells. Cultures were used undiluted or diluted 3-, 9-, or 27-fold (top to bottom) and assayed in the standard 51Cr release assay for 5 h. Bold values are significant above relevant controls (p < 0.1). Numbers in parentheses are % of lymphocytes of the indicated H-2.
Alloreactive CTL activity lysed infected and uninfected targets comparably.