Table 3.
The capacity of MHC-deficient murine thymi or rat thymi to reconstitute CD8+ T cell maturation and effector function in nude mice
| Recipient | Donor Thymus | Specific lysis of target cells, (%)
|
|
|---|---|---|---|
| MC57 (H-2b)
| |||
| LCMV | Uninf. | ||
| Nude (H-2b) | MHC | 72 | <1 |
| C57BL/6 | Class I0/0 | 74 | <1 |
| Class II0/0 | 50 | <1 | |
| (H-2b) | 47 | <1 | |
| Nude (H-2b) | Lewis | 80 | 7 |
| C57BL/6 | (Rat)0 | 54 | 3 |
| 31 | 2 | ||
| 19 | 1 | ||
| Nude (H-2b) | None | 5 | 3 |
| Control: | |||
| C57BL/6 | None | 85 | 6 |
Control values for LCMV-immune but H-2 incompatible effector CTLs or normal spleen cells were below 10% on peptide loaded or infected or normal targets. One example of two to three tested similar thymically reconstituted nude mice infected with 102 pfu of LCMV (8 days previously) is shown. Effector-to-target cell ratios were 90, 30, 10, 3:1 or 90:1. Bold values represent significant values above various controls (p < 0.1).
uninf., uninfected.