Table 4.

Maturation of tg TCR⁺ CD8⁺ T cells in nude mice: Dependence on host MHC

			Specific lysis of targets (%)
	CD4−CD8+ (%)†		MC57 H-2b		DBA1 H-2b
	CD4−CD8+V α 2+(%)		p33	Nor.	p118	Nor.
Mouse*	Naive blood	d7 LCMV spleen
nu/nu	<0.1	0.5	9	5	9	<1
TCR 318+	<0.1	0.1	3	9	7	<1
H-2q/q			<1	2	3	<1
			1	1	2	<1
nu/nu	<0.1	0.6	9	10	5	<1
TCR 318+	<0.1	0.1	7	10	7	<1
H-2q/q			6	5	4	<1
			5	7	5	<1
nu/nu	0.9	13.4	65	16	10	10
TCR 318+	0.7	12.8	55	14	14	6
H-2b/q			23	7	10	<1
			14	6	7	<1
nu/nu	1.5	9.4	73	17	16	18
TCR 318+	1.1	8.7	47	15	18	10
H-2b/q			25	6	10	6
			9	1	2	<1
Controls:
ICR	8.0	18.2	16	15	65	<1
H-2q/q	0.5	0.6	10	7	70	<1
C57BL/6	12.2	50.4	100	10	15	15
H-2b/b	1.1	4.5	93	10	16	18
TCR 318	15.4	28.4	44	12	14	15
H-2b/b	8	18	24	4	9	2

Significant values are bold. Nor., normal targets. 

^*Mice were infected i.v. with 10² pfu of LCMV-WE and CTL activity tested in a standard ⁵¹Cr release assay. Spontaneous release was <15% on MC57 and <20% on DBA 1 targets; the % lysis figures shown are for 90, 30, 10, and 3:1 or 90, 30:1 target cells from top to bottom. 

^†The results are expressed as % of living nucleated cells and are representative of 8 H-2^q/q and 10 H-2^b/q nude tg TCR⁺ mice tested. LCMV immune spleen cells from euthymic nu/+TCR 318 H-2^q/q mice lysed p 33 MC57 targets tested at 5, 1, 1, or 1% vs. normal MC57 6, 4, 1, or 1%, p118 DBA1 targets to 91, 92, 67, 42% vs. normal DBA1 targets 11, 18, 17, or 16%. Thus there is no relevant positive selection of functional tgTCR on H-2^q (see also Fig. 1).