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Stability of EGF-induced transcript. Cells were treated for 2 h with EGF and then incubated with 5 μg/ml actinomycin D for the indicated times. At the end of the incubation, total RNA was extracted and reverse-transcribed, and the cDNA was amplified with primers complementary to Dio2 and β-actin genes. Top, Ethidium bromide staining; bottom, densitometric analysis. Bars represent the Dio2/β-actin ratio.
