Fig. 3.

CD4⁺, CD8⁺ and CD25⁺ cell influx into pancreatic islets. Female mice (4 weeks old) were inoculated intramuscularly (i.m.) with three 100-μg doses of DNA-HSP65 (pVAX-HSP65) or DNAv (pVAX) at 15-day intervals. Controls received identical injections of phosphate-buffered saline (PBS). Pancreatic islets of 28-week-old mice were analysed. At least 20 islets from three sections per pancreas were examined. Samples from control group mice (a, b and c), DNA-HSP65 group mice (d, e and f) and DNAv group mice (g, h and i) were immunostained for CD4 (a, d and g), CD8 (b, e and h) and CD25 receptors (c, f and i). Original magnification, ×200; insets show the panoramic views (×40).