Fig. 4.

Comparable T cell priming and interferon (IFN)-γ production between IgM-deficient and wild-type mice. Wild-type (black bars) or IgM-deficient (open bars) mice were immunized with CII or chicken γ-globulin (CGG). Draining lymph node cells were isolated 7 days after immunization and stimulated with 10 µg/ml CII or CGG for 3 days. (a) Antigen-specific cellular proliferation in vitro. Cells were harvested 72 h later in the presence of [³H]-thymidine for the last 18 h. (b) IFN-γ production in supernatant was analysed by enzyme-linked immunosorbent assay at 3 days after in vitro re-stimulation with antigen. Data (mean ± standard error) are representative of three independent experiments with three to six mice in each group.