Fig. 1.
Identification of monocytes and dendritic cells (DC) by flow cytometry. A representative example of the identification of monocytes based on the expression of (a) CD33 and (b) CD14 antigen. (c) Identification of DC as a human leucocyte antigen D-related (HLA-DR)+ lineage– (CD3–, CD56–, CD14–, CD19–) population. (d) After acquiring a higher number of cells within the HLA-DR+ lineage– live gate, three different dendritic cell subsets were identified on the basis of CD33 expression: myeloid CD33high, CD33intermed and plasmocytoid CD33–/low.