Fig. 2.

Production of intracellular interleukin (IL)-4, IL-10 and IL-12 cytokines in bone marrow-derived dendritic cells (BMDC). BMDC were either unpulsed or pulsed with rV (10 µg/ml) + rF1 (5 µg/ml) for 24 h. BMDC were taken from cultures at 3 days (a) or 8 days (b). The open histogram represents the test sample with monensin; the shaded area shows the positive percentage of events when the control sample (no monensin) is compared and subtracted from the test sample. The boxed figures show the enhanced normalized subtraction (ENS) percentages of these positive values. Kolmogorov–Smirnov statistical analysis was used to assess the significance of this positivity. Production of IL-4, IL-10 and IL-12 were significant (P < 0.01). At day 13 (not shown) only the increase of IL-12 obtained in pulsed BMDC was significant (P < 0.01).