Fig. 4.

WY14,643 feeding does not alter IgG binding to the glomerular basement membrane (GBM). C57BL/6 mice were fed 0·05% WY14,643 or control food and then immunized to induce anti-glomerular basement membrane disease (AGBMD). Six months after induction of disease, kidneys were fixed and stained with fluorescein isothiocyanate-anti-mouse IgG. (a) Representative glomerulus. (b) Sections were graded qualitatively on a scale from 0 to 4 and there was no difference in the two treatment groups. (c) Fluorescence was measured quantitatively and there was no difference in IgG binding to the GBM. AU: arbitrary units; n.s.: not significant; N = 24.