Fig. 6.

WY14,643 alters the IgG1 and IgG response to α3(IV) NC1. Six months after induction of anti-glomerular basement membrane disease (AGBMD) sera was isolated and tested for anti-α3(IV) NC1-specific antibodies. These antigen-specific IgG enzyme-linked immunosorbent assays (ELISAs) generate relative curves that are not quantitative. Serum from a naive, unimmunized C57BL/6 mouse was also evaluated by ELISA and the horizontal dashed-lines represent the background of this assay (optic density of naive mouse serum). There was a significant increase in titres of antigen-specific IgG1 and IgG with a trend for a decline in titres of IgG2c in the WY14,643-treated mice. Also the IgG1/IgG2c ratio was significantly higher in the PPARα ligand-treated animals suggesting that WY14,643 induces a Th2-type of immune response. Values were assessed by analysis of variance with repeated measures and bars represent standard error of the mean (N = 27).