Fig. 8.

Mice treated with WY14,643 have lower renal expression of monocyte chemoattractant protein (MCP)-1. Mice were immunized to induce anti-glomerular basement membrane disease (AGBMD) and kidneys were harvested (a) 2 months (N = 8) and (b) 6 months after immunization (N = 20). RNA was prepared from kidneys and real-time polymerase chain reaction determined relative MCP-1/CCL2 mRNA expression. At both time-points there was a significant reduction in the expression of MCP-1 mRNA. (c) Five months after induction of AGBMD, urine was collected and MCP-1 protein levels were evaluated by enzyme-linked immunosorbent assay. There was a significant difference in MCP-1 urine values in control compared with WY14,643-treated mice (N = 29).