Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The principle of the anti-gliadin peptide dual-label immunofluorometric assay (IFMA) assay. The C-terminally biotinylated gamma gliadin peptide (residues 86–103) is attached to a streptavidin-coated microtitre plate. After incubation with serum sample and subsequent washes the plate is developed using secondary Europium-labelled anti-human IgA and Samarium-labelled anti-human IgG antibodies. The fluorescence is measured in a time-resolved fluorometer (Wallac).
